Quantitative Proteomics
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155 Federal Street, Suite 700, Boston
Isobaric Tag for Relative Absolute Quantitation (iTRAQ) and Tandem Mass Tags (TMT) are two similar quantitative proteomic techniques developed by AB SCIEX and Thermo Fisher respectively. The most distinctive advantage of these two techniques is that they can label up to 10 different samples and compare the relative proteomic change through LC-MS/MS analysis. Therefore these techniques are widely applied to study proteomic changes under different treatments or in different development stages. MtoZ Biolabs is proud to offer iTRAQ/TMT analysis service to meet your research needs. On the basis of these two well-established techniques we can also provide MultiNotch MS analysis... read more
Isobaric Tag for Relative Absolute Quantitation (iTRAQ) and Tandem Mass Tags (TMT) are two similar quantitative proteomic techniques developed by AB SCIEX and Thermo Fisher respectively. The most distinctive advantage of these two techniques is that they can label up to 10 different samples and compare the relative proteomic change through LC-MS/MS analysis. Therefore these techniques are widely applied to study proteomic changes under different treatments or in different development stages. MtoZ Biolabs is proud to offer iTRAQ/TMT analysis service to meet your research needs. On the basis of these two well-established techniques we can also provide MultiNotch MS analysis... read more
155 Federal Street, Suite 700, Boston
Similar to SILAC technique Dimethyl labeling is also an isotopic labeling method. However Dimethyl labeling is a chemical labeling process and labels lysine or the N-terminal amino acid residues on peptide instead of labeling proteins. Except for the difference in labeling method SILAC and Dimethyl labeling technique have comparable sensitivity and accuracy and are being more and more widely used in relative proteomics studies. MtoZ Biolabs has developed a specialized platform equipped with Q Exactive HF (Thermo Fisher) Orbitrap Fusion and Orbitrap Fusion Lumos mass spectrometers equipped with Nano-LC for SILAC/Dimethyl analysis service.... read more
Similar to SILAC technique Dimethyl labeling is also an isotopic labeling method. However Dimethyl labeling is a chemical labeling process and labels lysine or the N-terminal amino acid residues on peptide instead of labeling proteins. Except for the difference in labeling method SILAC and Dimethyl labeling technique have comparable sensitivity and accuracy and are being more and more widely used in relative proteomics studies. MtoZ Biolabs has developed a specialized platform equipped with Q Exactive HF (Thermo Fisher) Orbitrap Fusion and Orbitrap Fusion Lumos mass spectrometers equipped with Nano-LC for SILAC/Dimethyl analysis service.... read more
155 Federal Street, Suite 700, Boston
Tel: 16174018103
"Proteomics is a large-scale study of proteins in a certain organism. Depending on specific research objective there are many analytical techniques for proteomics study. To better meet our clients’ needs MtoZ Biolabs is proud to offer 5 quantitative proteomics service including Label-free iTRAQ/TMT SILAC MRM and SWATH. We also provide custom proteomics service for specific project. You are welcome to contact us for more detailed information on project design and sample requirements. Service Category: label-free proteomics iTRAQ/TMT SILAC/Dimethyl SWATH MRM... read more
Tel: 16174018103
"Proteomics is a large-scale study of proteins in a certain organism. Depending on specific research objective there are many analytical techniques for proteomics study. To better meet our clients’ needs MtoZ Biolabs is proud to offer 5 quantitative proteomics service including Label-free iTRAQ/TMT SILAC MRM and SWATH. We also provide custom proteomics service for specific project. You are welcome to contact us for more detailed information on project design and sample requirements. Service Category: label-free proteomics iTRAQ/TMT SILAC/Dimethyl SWATH MRM... read more
155 Federal Street, Suite 700, Boston
Tel: 16174018103
"Label-free analysis is a powerful technique for identifying and quantifying relative changes in complex protein samples. In this method samples are run individually and identified by MS/MS information. Relative quantitation of different samples is then analyzed by aligning chromatographic peak areas of precursor ions between various runs. The most distinctive advantage is that it quantifies proteins without any use of labels and can analyze unlimited number of samples. Since Label-free is relatively simple and easy compared to other proteomics techniques it has been widely used for proteomics study and biomarker discovery. MtoZ Biolabs is proud to offer Label-free quantitative proteomics... read more
Tel: 16174018103
"Label-free analysis is a powerful technique for identifying and quantifying relative changes in complex protein samples. In this method samples are run individually and identified by MS/MS information. Relative quantitation of different samples is then analyzed by aligning chromatographic peak areas of precursor ions between various runs. The most distinctive advantage is that it quantifies proteins without any use of labels and can analyze unlimited number of samples. Since Label-free is relatively simple and easy compared to other proteomics techniques it has been widely used for proteomics study and biomarker discovery. MtoZ Biolabs is proud to offer Label-free quantitative proteomics... read more
155 Federal Street, Suite 700, Boston
Sequential Window Acquisition of all Theoretical fragment ions (SWATH) is a brand new mass spectrometry technology co-developed by Dr. Ruedi Aebersold research group (ETH Zürich) and AB SCIEX. SWATH data is an independent acquisition (DIA) technique. Unlike data dependent acquisition technique which only selects certain MS spectrum for MS/MS detection SWATH analyzes all MS and MS/MS spectra allowing detection of virtually all detectable proteins in a sample. In SWATH technology MS spectra are divided into several narrow windows and peptides in each window are sequentially analyzed equally followed by MS/MS analysis. SWATH technique combines the high-throughput quality of shotgun proteomics... read more
Sequential Window Acquisition of all Theoretical fragment ions (SWATH) is a brand new mass spectrometry technology co-developed by Dr. Ruedi Aebersold research group (ETH Zürich) and AB SCIEX. SWATH data is an independent acquisition (DIA) technique. Unlike data dependent acquisition technique which only selects certain MS spectrum for MS/MS detection SWATH analyzes all MS and MS/MS spectra allowing detection of virtually all detectable proteins in a sample. In SWATH technology MS spectra are divided into several narrow windows and peptides in each window are sequentially analyzed equally followed by MS/MS analysis. SWATH technique combines the high-throughput quality of shotgun proteomics... read more
155 Federal Street, Suite 700, Boston
Bioamine is a kind of low molecular weight compounds containing nitric fatty group or heterocycle group. Depending on the composition of amine bioamine can be divided into two classes monoamine and polyamine. Monoamine plays a role in muscular and blood vessel relaxation and regulates cerebral cortex activity. Polyamine mainly promotes synthesis of macromolecules including DNA RNA and proteins and accelerates development and growth. MtoZ Biolabs offers targeted bioamine analysis service using ACQUITY UPLC/TripleQuad5500 (Waters/AB Sciex) which have characteristics of high accuracy specificity and sensitivity. We guarantee accurate analysis of bioamine even in low abundance. With our optimized sample preparation methods... read more
Bioamine is a kind of low molecular weight compounds containing nitric fatty group or heterocycle group. Depending on the composition of amine bioamine can be divided into two classes monoamine and polyamine. Monoamine plays a role in muscular and blood vessel relaxation and regulates cerebral cortex activity. Polyamine mainly promotes synthesis of macromolecules including DNA RNA and proteins and accelerates development and growth. MtoZ Biolabs offers targeted bioamine analysis service using ACQUITY UPLC/TripleQuad5500 (Waters/AB Sciex) which have characteristics of high accuracy specificity and sensitivity. We guarantee accurate analysis of bioamine even in low abundance. With our optimized sample preparation methods... read more
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