Protein
178 RESULTS WITHIN 50km FROM Burlington, United States
80 Orville Dr. Suite 100-10131, Bohemia
Tel: 06316197922
Creative Diagnostics provides a protocol for total protein extraction based on RIPA which is widely used.... read more
Tel: 06316197922
Creative Diagnostics provides a protocol for total protein extraction based on RIPA which is widely used.... read more
80 Orville Dr. Suite 100-10131, Bohemia
Tel: 06316197922
This protocol provides a detailed procedure for total protein extraction for western blot analysis from achieved samples following TRIzol isolation of RNA.... read more
Tel: 06316197922
This protocol provides a detailed procedure for total protein extraction for western blot analysis from achieved samples following TRIzol isolation of RNA.... read more
45-1 Ramsey Road, Shirley, NY 11967, USA, Shirley
Tel: 6316197922
Protein extraction from tissues and cultured cells is the first step for many biochemical and analytical techniques such as protein purification western blotting as well as mass spectrometry. There is still no universal set of method or regent that is optimal for protein extraction due to the chemical/physical heterogeneity of proteins and their sample sources. Some factors should be taken into account when handling proteins such as sample types subcellular locations of the protein the compatibility of the protein as well as downstream applications. Here we provide a protocol for total protein extraction based on RIPA which is widely used.... read more
Tel: 6316197922
Protein extraction from tissues and cultured cells is the first step for many biochemical and analytical techniques such as protein purification western blotting as well as mass spectrometry. There is still no universal set of method or regent that is optimal for protein extraction due to the chemical/physical heterogeneity of proteins and their sample sources. Some factors should be taken into account when handling proteins such as sample types subcellular locations of the protein the compatibility of the protein as well as downstream applications. Here we provide a protocol for total protein extraction based on RIPA which is widely used.... read more
45-1 Ramsey Road, Shirley, New York 11967, USA, Shirley
Profacgen makes use of the most state-of-the-art protein–ligand docking software tools to predict the position and orientation of a ligand when it is bound to a protein receptor by calculating the site geometry and energy. The process of docking a ligand to a binding site mimics the natural course of interaction of the ligand and its receptor via the lowest energy pathway. Typically our modeling procedures starts with a target of known structure such as a crystallographic structure of a protein of interest. Docking is then used to predict the bound conformation and binding free energy of small molecules to... read more
Profacgen makes use of the most state-of-the-art protein–ligand docking software tools to predict the position and orientation of a ligand when it is bound to a protein receptor by calculating the site geometry and energy. The process of docking a ligand to a binding site mimics the natural course of interaction of the ligand and its receptor via the lowest energy pathway. Typically our modeling procedures starts with a target of known structure such as a crystallographic structure of a protein of interest. Docking is then used to predict the bound conformation and binding free energy of small molecules to... read more
45-1 Ramsey Road, Shirley, New York 11967, USA, Shirley
Protein–protein docking is the computational prediction of protein complex structure as it would occur in a living organism given the individually solved component protein structures. Profacgen makes use of state-of-the-art docking software tools to find the relative transformation and conformation of two proteins that result in a stable (energetically favorable) complex. The docking process is composed of two main steps: 1) Generating a set of configurations which reliably includes at least one nearly correct one and 2) reliably distinguishing nearly correct configurations from the others with a scoring function. Our procedure starts with rigid body global search based on the... read more
Protein–protein docking is the computational prediction of protein complex structure as it would occur in a living organism given the individually solved component protein structures. Profacgen makes use of state-of-the-art docking software tools to find the relative transformation and conformation of two proteins that result in a stable (energetically favorable) complex. The docking process is composed of two main steps: 1) Generating a set of configurations which reliably includes at least one nearly correct one and 2) reliably distinguishing nearly correct configurations from the others with a scoring function. Our procedure starts with rigid body global search based on the... read more
45-1 Ramsey Road, Shirley, New York 11967, USA, Shirley
Profacgen makes use of state-of-the-art docking software tools to model protein–peptide interactions and to predict the complex structure. The general modeling procedure is divided into two stages: (1) a global search is performed to predict the binding site at the protein receptor surface; (2) a flexible local docking method is used to fit the peptide structure into the known binding site. These two steps take into consideration the significant conformational changes upon binding of the two components. The peptide folding process is explicitly simulated in the docking protocol. When experimental or predicted data on binding site residues are available such... read more
Profacgen makes use of state-of-the-art docking software tools to model protein–peptide interactions and to predict the complex structure. The general modeling procedure is divided into two stages: (1) a global search is performed to predict the binding site at the protein receptor surface; (2) a flexible local docking method is used to fit the peptide structure into the known binding site. These two steps take into consideration the significant conformational changes upon binding of the two components. The peptide folding process is explicitly simulated in the docking protocol. When experimental or predicted data on binding site residues are available such... read more
45-1 Ramsey Road, Shirley, New York 11967, USA, Shirley
Protein–lipid interactions are stabilized by the formation of intermolecular hydrogen bonds van der Waals interactions hydrophobic interactions and ionic bridges (especially aspartate or glutamate residues) between the protein and lipid ligand. Profacgen makes use of the state-of-the-art computational software tools to predict these protein–lipid interactions. The lipid binding sites of a protein can be deduced from its amino acid sequence and/or predicted from its three-dimensional structure using molecular docking protocols. Our docking method combines sequence and structure information and explores the most energetically favorable protein-lipid complex. The scoring function is specifically designed to allow for the prediction of lipid distortion... read more
Protein–lipid interactions are stabilized by the formation of intermolecular hydrogen bonds van der Waals interactions hydrophobic interactions and ionic bridges (especially aspartate or glutamate residues) between the protein and lipid ligand. Profacgen makes use of the state-of-the-art computational software tools to predict these protein–lipid interactions. The lipid binding sites of a protein can be deduced from its amino acid sequence and/or predicted from its three-dimensional structure using molecular docking protocols. Our docking method combines sequence and structure information and explores the most energetically favorable protein-lipid complex. The scoring function is specifically designed to allow for the prediction of lipid distortion... read more
45-1 Ramsey Road, Shirley, New York 11967, USA, Shirley
At Profacgen we predict these protein–carbohydrate interactions through the combination of a genetic conformational search algorithm coupled with an empirical free energy function specific for carbohydrates. Our docking procedure allows for a systematic exploration of the orientations and positions of carbohydrate ligand into a protein cavity (which is usually more open and less well defined compared to small molecule binding sites) and assesses free energies of complex formation. Experiment-derived constraints can also be applied to the docking protocol to restrict the search space for the sugar as well as the protein molecule. The energy function is specifically designed for carbohydrates... read more
At Profacgen we predict these protein–carbohydrate interactions through the combination of a genetic conformational search algorithm coupled with an empirical free energy function specific for carbohydrates. Our docking procedure allows for a systematic exploration of the orientations and positions of carbohydrate ligand into a protein cavity (which is usually more open and less well defined compared to small molecule binding sites) and assesses free energies of complex formation. Experiment-derived constraints can also be applied to the docking protocol to restrict the search space for the sugar as well as the protein molecule. The energy function is specifically designed for carbohydrates... read more
8878 Covenant Ave. #123, Pittsburgh
Tel: 18448385227
MIracle Vet makes high-quality dog food and supplements for pets. Our food is veterinarian designed and formulated. Our all natural products include: High-quality dog food Omega probiotics chews High-calorie chews Dog and cat weight gainer And on the go protein bars Our products do not contain any wheat gain or soy and are 100% all natural and made in our FDA approved facility.... read more
Tel: 18448385227
MIracle Vet makes high-quality dog food and supplements for pets. Our food is veterinarian designed and formulated. Our all natural products include: High-quality dog food Omega probiotics chews High-calorie chews Dog and cat weight gainer And on the go protein bars Our products do not contain any wheat gain or soy and are 100% all natural and made in our FDA approved facility.... read more
4885-A McKnight Road #413, Pittsburgh
Tel: +1 844-285-5987
Bully Max makes high-quality dog food and supplements for all dog breeds. Bully Max supplements are safe for all dogs breeds and 100% natural and made in an FDA approved facility. Bully Max is trusted by vets k9 champion breeders and k9 police units all over the world. ANd helps to improve your dog's recovery health and energy. Bully Max can also help with your dog's size and muscle. Bully Max products are high-calorie dog food Bully Max original Gorilla Max and protein chews.... read more
Tel: +1 844-285-5987
Bully Max makes high-quality dog food and supplements for all dog breeds. Bully Max supplements are safe for all dogs breeds and 100% natural and made in an FDA approved facility. Bully Max is trusted by vets k9 champion breeders and k9 police units all over the world. ANd helps to improve your dog's recovery health and energy. Bully Max can also help with your dog's size and muscle. Bully Max products are high-calorie dog food Bully Max original Gorilla Max and protein chews.... read more
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